Recombinante rat Cystatine C-eiwit

CST3, also known as cystain-C, is secreted type 2 cysteine proteinase inhibitors of the cystatin superfamily. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. Recombinant rat CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Cystain-C, CST3, CYSC

Baculovirus

Rat

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

14.3 kDa (128aa)

Cystatin C, Cystain-C, CST3, CYSC, ATGP3313-10 µg, ATGP3313-20 µg, ATGP3313-50 µg, ATGP3313-100 µg, ATGP3313-250 µg, ATGP3313-500 µg, ATGP3313-1 mg, ATGP3313-10, ATGP3313-20, ATGP3313-50, ATGP3313-100, ATGP3313-250, ATGP3313-500, ATGP3313-1

Reed CH., et al. (2000) Br J Biomed. Sci. 57:323-329.; ; Esnard A., et al. (1992) FEBS Lett. 300:131-135.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cancer

NP_036969

P14841

Rat

Gly21

GTSRPPPRLL GAPQEADASE EGVQRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL TNCPFHDQPH LMRKALCSFQ IYSVPWKGTH TLTKSSCKNA LEHHHHHH