REPS2 Antilichaam / RalBP1-geassocieerd Eps domein-bevattend eiwit 2

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q8NFH8

Mouse, Rat

E.coli-derived human REPS2 recombinant protein (Position: A76-K564) was used as the immunogen for the REPS2 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This REPS2 antibody is available for research use only.

After reconstitution, the REPS2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the REPS2 antibody should be determined by the researcher.

Western blot analysis of REPS2 using anti-REPS2 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat brain tissue lysates, Lane 2: rat NRK whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REPS2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for REPS2 at approximately 72 kDa. The expected molecular weight of REPS2 is ~72 kDa.