RFC5 Antilichaam - N-terminalgebied: HRP (ARP46172 P050-HRP)

Replication factor C (activator 1) 5, 36.5kDa

RFC36

5985

P40937

NP_031396

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Yeast, Zebrafish

The immunogen is a synthetic peptide directed towards the N terminal region of human RFC5

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

NAB2; XIAP; TRIM38; HUWE1; RFC4; SUMO2; PCNA; UBC; RPA3; RPA2; RPA1; EED; FBXO6; MMS19; VCP; ECT2; MDC1; MTMR2; RFC3; RFC2; RFC1; CAND1; CUL3; NEDD8; HDGF; SMARCAD1; PIDD1; MYC; RPAP3; MEPCE; RUVBL2; LRIF1; COPS6; MED31; RBM48; UNC119; EEF1A1; RAD17; BRD4

HRP: Horseradish Peroxidase

Polyclonal Antibody

IHC, WB

Affinity Purified

RFC5 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

0.5 mg/ml

Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rat: 100%; Yeast: 90%; Zebrafish: 93%

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

38kDa

Ohta, S., (2002) J. Biol. Chem. 277 (43), 40362-40367

Wet Ice

340

RFC5

RFC5

Rabbit

Replication factor C subunit 5

NM_007370

METSALKQQEQPAATKIRNLPWVEKYRPQTLNDLISHQDILSTIQKFINE

5