RFX4 Antilichaam / Regelgevende factor X4

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q33E94

Human

E.coli-derived human RFX4 recombinant protein (Position: Q124-H667) was used as the immunogen for the RFX4 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This RFX4 antibody is available for research use only.

After reconstitution, the RFX4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the RFX4 antibody should be determined by the researcher.

Western blot analysis of RFX4 using anti-RFX4 antibody. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX4 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. RFX4 (~83 kDa predicted) was detected as multiple bands between 70 and 120 kDa, consistent with reported alternative splice variants and proteolytic fragments of RFX4 in glioma and brain-derived cells.