RHOT1 Antilichaam

The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1) . RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3) . Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4) .

55288

Q8IXI2

NP_001028740

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Neuroscience

RHOT1 antibody is affinity chromatography purified via peptide column.

Cat. No. 1463 - Rat Brain Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (81%)

RHOT1 antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

RHOT1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

IgG

RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist; this antibody will detect all three isoforms.

RHOT1

Ras homolog family member T1

Homo sapiens

Fransson A, Ruusala A, and Aspenstrom P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. J. Biol. Chem. 2003; 278:6495-502.

Macaskill AF, Brickley K, Stephenson FA, et al. GTPase dependent recruitment of Grif-1 by Miro1 regulates mitochondrial trafficking in hippocampal neurons. Mol. Cell. Neurosci. 2009;40:301–12.

Weihofen A, Thomas KJ, Ostaszewski B, et al. Pink1 forms a multi-protein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking. Biochemistry 2009; 48:2045-54.

Wang X, Winter D, Ashrafi G, et al. PINK1 and Parkin target Miro for phosphorylation and degradation to arrest mitochondrial motility. Cell 2011; 147:893-906.

Ras homolog family member T1, mitochondrial Rho GTPase 1, ARHT1, MIRO-1, MIRO1, MIRO

ELISA, WB, IHC-P, IF

75750480

Liquid