RIP3 Antilichaam

RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κ B activation. Overexpression of RIP3 induces apoptosis and NF-κ B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.

56532

Q9QZL0

AAF03133

Human, Mouse, Rat

2283

Unconjugated

Primary Antibodies

KO Validation (Figure 7,9,13) shows RIP3 expression detected by anti-RIP3 antibodies (2283) was disrupted in multiple tissues of RIP3 KO mice (figure 7, 13) and in RIP3 KO MEF cells (figure 9) . KO Validation KD validation (Figure 10) : Anti-RIP3 antibody (2283) specificity was further verified by RIP3 specific knockdown. RIP3 signal in L929 cells transfected with RIP3 shRNA was disrupted in comparison with that in cells transfected with control shRNA. KD validation2283 Regulated expression validation (Figure 7,11,13) : RIP3 expression detected by anit-RIP3 antibodies (2283) was up-regulated by treatment of cerulein (figure 7), LPS (figure 11) or acetaminophen (figure 13) in different tissues or cells. Regulated expression validation2283 Overexpression validation (Figure 12) : RIP3 overexpression detected by anit-RIP3 antibodies (2283) was observed in three cell lines stably transfected with RIP3. Overexpression validation2283

Apoptosis

RIP3 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1202 – A431 Cell Lysate

1 mg/ml

RIP3 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

RIP3 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

IgG

Mouse RIP3 has one isoform (486aa, 53kD) . Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57kD), isoform 2 (252aa, 28kD) and isoform 3 (231aa, 25kD) . Rat RIP3 also has one isoform (478aa, 52kD) . 2283 can detect can detect isoforms of mouse and rat as well as human isoform 1.

Ripk3

Cat. No. 1282 - 3T3/NIH Cell Lysate

Cat. No. 1285- C2C12 Cell Lysate

Cat. No. 1212- 3T3/Balb Cell Lysate

Receptor-interacting serine-threonine kinase 3

Mus musculus

Yu et al. Curr Biol. 1999;9 (10) :539-42.

Sun et al. J Biol Chem. 1999;274 (24) :16871-5.

Pazdernik et al. Mol Cell Bio. 1999; 19 (10) :6500-8 (WD0102)

19524512

21368761

23201684

26943325

28981102

23744808

22817896

24441827

25326752

25939025

24012422

27321907

28085133

26418225

23734789

23348743

22262768

28871172

27532042

21402742

RIP3 Antibody: Rip3, AW107945, 2610528K09Rik, Rip3, RIP-like protein kinase 3, RIP-3

ELISA, IF, IHC-P, WB, IP

6063101

Liquid