RPP30 cDNA

RPP30 (Ribonuclease P protein subunit p30) belongs to the eukaryotic/archaeal RNase P protein component 3 family. RPP30 is component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ribonuclease P (PNase P) is small nuclear ribonucleoproteins (snRNPs) that act on RNA substrates in vitro. Also, RNase P accumulates in the nucleolus, have a similar RNA component and also have several protein subunits in common.

TSG15

10q23.31

Human

PATGen (puc19-derived cloning vector)

ATGGCGGTGT TTGCAGATTT GGACCTGCGA GCGGGTTCTG ACCTGAAGGC TCTGCGCGGA CTTGTGGAGA CAGCCGCTCA CCTTGGCTAT TCAGTTGTTG CTATCAATCA TATCGTTGAC TTTAAGGAAA AGAAACAGGA AATTGAAAAA CCAGTAGCTG TTTCTGAACT CTTCACAACT TTGCCAATTG TACAGGGAAA ATCAAGACCA ATTAAAATTT TAACTAGATT AACAATTATT GTCTCGGATC CATCTCACTG CAATGTTTTG AGAGCAACTT CTTCAAGGGC CCGGCTCTAT GATGTTGTTG CAGTTTTTCC AAAGACAGAA AAGCTTTTTC ATATTGCTTG CACACATTTA GATGTGGATT TAGTCTGCAT AACTGTAACA GAGAAACTAC CATTTTACTT CAAAAGACCT CCTATTAATG TGGCGATTGA CCGAGGCCTG GCTTTTGAAC TTGTCTATAG CCCTGCTATC AAAGACTCCA CAATGAGAAG GTATACAATT TCCAGTGCCC TCAATTTGAT GCAAATCTGC AAAGGAAAGA ATGTAATTAT ATCTAGTGCT GCAGAAAGGC CTTTAGAAAT AAGAGGGCCA TATGACGTGG CAAATCTAGG CTTGCTGTTT GGGCTCTCTG AAAGTGACGC CAAGGCTGCG GTGTCCACCA ACTGCCGAGC AGCGCTTCTC CATGGAGAAA CTAGAAAAAC TGCTTTTGGA ATTATCTCTA CAGTGAAGAA ACCTCGGCCA TCAGAAGGAG ATGAAGATTG TCTTCCAGCT TCCAAGAAAG CCAAGTGTGA GGGCTGA

RPP30, TSG15, RPP30, ATGD0038-10 µg, ATGD0038-20 µg, ATGD0038-50 µg, ATGD0038-100 µg, ATGD0038-250 µg, ATGD0038-500 µg, ATGD0038-1 mg, ATGD0038-10, ATGD0038-20, ATGD0038-50, ATGD0038-100, ATGD0038-250, ATGD0038-500, ATGD0038-1

Store the plasmid at -20C.

Lyophilized

Metabolism

606115

NP_006404.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_006413.4

807bp

This shuttle vector contains the complete ORF. It is inseted Kpn I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.