RPS17 cDNA

RPS17 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in RPS17 cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS17 dispersed through the genome

DBA4, RPS17L, RPS17L1, RPS17L2, S17

15q

Human

PATGen (puc19-derived cloning vector)

ATGGGCCGCGTTCGCACCAAAACCGTGAAGAAGGCGGCCCGGGTCATCATAGAAAAGTACTACACGCGCCTGGGCAACGACTTCCACACGAACAAGCGCGTGTGCGAGGAGATCGCCATTATCCCCAGCAAAAAGCTCCGCAACAAGATAGCAGGTTATGTCACGCATCTGATGAAGCGAATTCAGAGAGGCCCAGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGGAGAGACAATTATGTTCCTGAGGTCTCAGCCTTGGATCAGGAGATTATTGAAGTAGATCCTGACACTAAGGAAATGCTGAAGCTTTTGGACTTCGGCAGTCTGTCCAACCTTCAGGTCACTCAGCCTACAGTTGGGATGAATTTCAAAACGCCTCGGGGACCTGTTTGA

RPS17, DBA4, RPS17L, RPS17L1, RPS17L2, S17, RPS17, ATGD0478-10 µg, ATGD0478-20 µg, ATGD0478-50 µg, ATGD0478-100 µg, ATGD0478-250 µg, ATGD0478-500 µg, ATGD0478-1 mg, ATGD0478-10, ATGD0478-20, ATGD0478-50, ATGD0478-100, ATGD0478-250, ATGD0478-500, ATGD0478-1

Store the plasmid at -20C.

Lyophilized

Epigenetics and Nuclear Signaling

180472

NP_001012.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001021.4

408bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.