RYK-antilichaam

RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.

Western blot: 1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

P34925

Human, Mouse

A portion of amino acids 160-190 from the human protein were used as the immunogen for the RYK antibody.

WB, IHC-P

Antigen affinity purified

Purified

This RYK antibody is available for research use only.

Aliquot the RYK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the RYK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Western blot testing of human ovary lysate with RYK antibody. Predicted molecular weight ~68 kDa.