S. Japonicum Glutathion S-transferase/GST Antilichaam

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST proteins can be purified by immobilized glutathione affinity chromatography. Many recombinant proteins have been engineered with GST tags to facilitate the detection, isolation and purification of these proteins. Monoclonal antibody reacting specifically with GST may be useful in various immunotechnique, to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors.

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

Schistosoma japonicum

Recombinant Schistosoma japonicum Glutathione S transferase (GST) purified from E. coli

100000

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

GST, AGT0501-10 µg, AGT0501-20 µg, AGT0501-50 µg, AGT0501-100 µg, AGT0501-250 µg, AGT0501-500 µg, AGT0501-1 mg, AGT0501-10, AGT0501-20, AGT0501-50, AGT0501-100, AGT0501-250, AGT0501-500, AGT0501-1

Smith, D., et al., Proc. Natl. Acad. Sci. USA, 83, 8703 (1986) .; ; Cartwright, G., et al., J. Immunol. Meth., 179, 31 (1995) .; ; Hibma, M., et al., Nucl. Ac. Res., 22, 3806 (1994) .

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Protein Marker

AAB59203.1

P08515

The recombinant protein GST (50ng,28kDa) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GST antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The recombinant human GST-fusion protein (50ng,49kDa) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GST antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; {2004010011202255.jpg}

ICC/IF analysis of GST in HeLa cells. The cell was stained with AGT0501 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .