S100A4 Antilichaam / S100 calciumbindend eiwit A4

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, α6β4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

P26447

Human

A portion of amino acids 1-101 from human S100A4 protein was used as the immunogen for the S100A4 antibody.

S100A4/6802

IHC-P, WB

Protein G affinity

Purified

This S100A4 antibody is available for research use only.

Aliquot the S100A4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the S100A4 antibody should be determined by the researcher.

Cytoplasm, Nucleus

IHC staining of FFPE human spleen tissue with S100A4 antibody (clone S100A4/6802) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.