S100A4 Antilichaam / S100 calciumbindend eiwit A4

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, α6β4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

P26447

Human

A portion of amino acids 1-101 from human S100A4 protein was used as the immunogen for the S100A4 antibody.

S100A4/6801

IHC-P, WB

Protein G affinity

Purified

This S100A4 antibody is available for research use only.

Aliquot the S100A4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the S100A4 antibody should be determined by the researcher.

Cytoplasm, Nucleus

IHC staining of FFPE human spleen tissue with S100A4 antibody (clone S100A4/6801) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.