S1P1 Antilichaam

S1P1 Antibody: Movement of lymphocytes through lymphoid organs is required for generating immunity. Their migration into lymph nodes follows a series of events including integrin activation through chemokine signaling, adhesion and diapedis. The release of lymphocytes from lymph nodes is regulated by the phospholipid sphingosine-1-phosphate (S1P) . One of its receptors S1P1 binds S1P with high specificity and affinity; agonism of this receptor by the immunosuppressive agent FTY720 inhibits the entry of tissue T cells into afferent lymphatics in homeostatic and inflammatory conditions. Recent experiments have indicated that CCR7-deficient T cells left lymph nodes more rapidly than wild-type cells did and these cells where also less effectively retained after treatment with FTY720, and that egress competence could be restored by inactivating G alpha i-protein-coupled receptor signaling. These results suggest that S1P1 acts in the lymphocyte to promote lymph node egress by overcoming retention signals mediated by CCR7 and G alpha i-protein-coupled receptor signaling.

1901

P21453

NP_001391

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Immunology

S1P1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1409 - Mouse Thymus Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Bovine: (86%)

S1P1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

S1P1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

At least two isoforms of S1P1 are known to exist; this S1P1 antibody will only recognize the shorter isoform.

S1PR1

Sphingosine-1-phosphate receptor 1

Homo sapiens

Miyasaka M and Tanaka T. Lymphocyte tracking across high endothelial venules: dogmas and enigmas. Nat. Rev. Immunol.2004; 3:360-70.

Mandala S, Hajdu R, Bergstrom J, et al. Alteration of lymphocyte tracking by sphingosine-1-phosphate receptor agonists. Science 2002; 296:346-9.

Ledgerwood LG, Lal G, Zhang N, et al. The sphingosine-1-phosphate receptor 1 causes tissue retention by inhibiting the entry of peripheral tissue T lymphocytes into afferent lymphatics. Nat. Immunol. 2008; 9:42-53.

Pham TH, Okada T, Matloubian M, et al. S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress. Immunity 2008; 28:122-33.

S1P1 Antibody: EDG1, S1P1, CD363, ECGF1, EDG-1, CHEDG1, D1S3362, EDG1, Sphingosine 1-phosphate receptor 1, Endothelial differentiation G-protein coupled receptor 1, S1P receptor 1

ELISA, WB, IHC-P, IF

13027636

Liquid