SAMHD1 (fosfo Thr592) Antilichaam

The SAM domain and HD domain 1 (SAMHD1) protein is upregulated in response to viral infection and is thought to play a role in innate immunity (1) . SAMHD1 blocks the infection of HIV-1 and SIVdeltaVpx before reverse transcription in macrophages and dendritic cells (2), and this restriction is regulated by phosphorylation of SAMHD1 (3) . Mutations in this gene have been associated with Aicardi-Goutieres syndrome (1) .

25939

Q9Y3Z3

NP_056289

Human, Mouse

8005

Unconjugated

Primary Antibodies

Mutant validation (Figure 2, 5, 6 & 7) : Anti-SAMHD1 (phospho Thr592) antibody (8005) specificity was further verified by SAMHD1 (phospho Thr592) mutant. SAMHD1 (phospho Thr592) signal in 293T cells (figure 2) or HEK293T cells (figure 5 & 7) or U937 cells (figure 6) transfected with SAMHD1 (phospho Thr592) mutant was disrupted in comparison with that in cells transfected with the wild-type. Mutant validation8005 KD validation (Figure 8) : SAMHD1 (phospho Thr592) expression detected by anit-SAMHD1 (phospho Thr592) antibodies (8005) was disrupted in knockdown macrophages. KD validation8005 Regulated expression validation (Figure 10) : SAMHD1 (phospho Thr592) expression detected by anit-SAMHD1 (phospho Thr592) antibodies (8005) was down-regulated by lambda-phosphatase treatment in S and G2 phase of cell cycle. Regulated expression validation8005

Immunology, Innate Immunity

SAMHD1 (phospho Thr592) antibody is affinity chromatography purified via peptide column.

Cat. No. 10-301 - Human Brain Tissue Slide

1 mg/mL

SAMHD1 (phospho Thr592) antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

SAMHD1 (phospho Thr592) antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

IgG

Phospho-SAMHD1 antibody will only detect the phosphorylated form of SAMHD1.

Human SAMHD1 (phospho Thr592) has 3 isoforms, including isoform 1 (626aa, 72kD), isoform 3 (556aa, 64kD), isoform 4 (591aa, 68kD) . Mouse SAMHD1 has 2 isoforms, including (658aa, 76kD) and (651aa, 75kD) . Rat SAMHD1 has only one isoform (620aa, 72kD) . 8005 can detect human and mouse.

SAMHD1 (phospho Thr592)

SAM domain and HD domain 1

Homo sapiens

Rice et al. Nat. Genet. 2009; 41:829-32.

Hrecka et al. Nature 2011; 474:654-7.

Welbourn et al. J. Virol. 2013; 87:11516-24.

27711056

30132758

29321329

30039733

28122869

29084722

29610582

30068654

27815502

26667483

Feifei Wang. Comparison of Murine and Human SAMHD1's Role in Retroviral Restriction and Cell Cycle Progression. PhD Thesis. The Ohio State University. 2015.

Alexandra Herrmann. The role of SAMHD1 in restriction and immune sensing of retroviruses and retroelements. PhD Thesis. The Friedrich Alexander University Erlangen-Nuremberg. 2018.

SAM domain and HD domain 1, DCIP, CHBL2, HDDC1, MOP-5, SBBI88

ELISA, WB, IHC, IF

38016914

Liquid