SAP97 Antilichaam, Kloon G28

SAP97 (Synapse-Associated Protein 97), a member of the MAGUK family, is a critical scaffolding protein involved in the trafficking and localization of glutamate receptors, which are essential for synaptic plasticity and cognitive function. Mutations in the DLG1 gene encoding SAP97 have been linked to schizophrenia, with studies showing that these mutations enhance glutamatergic synapse strength in the dentate gyrus and impair episodic memory processing. SAP97 also mediates a non-conventional secretory pathway for NMDA receptors via dendritic Golgi outposts, highlighting its unique role in receptor sorting and synaptic regulation. Furthermore, SAP97 regulates the trafficking of ADAM10, a key α-secretase involved in amyloid precursor protein processing, implicating it in Alzheimer’s disease mechanisms. Our Anti-Rat SAP97 recombinant monoclonal antibody has been demonstrated to detect the target in rodent brain lysates and in cell culture lines.

Rabbit Anti-Rat SAP97 Recombinant Monoclonal IgG Kappa

SAP97 Antibody, DLGH1 Antibody, DLGH 1 Antibody, DLG1 Antibody, DLG 1 Antibody, Synapse-associated protein 97 Antibody, Disks large homolog 1 Antibody, SAP-97 Antibody, SAP 97 Antibody

12352203

Non-hazardous

Non-hazardous

25252

Q62696

NP_036920.2

Fusion protein, AA #1-104 (N-terminus) of rat SAP97.

G28

Unconjugated

Widely expressed. Brain, Heart, Muscle, Lung, Liver, Epithelial Cells

Recombinant Monoclonal

WB | ICC-IF

Neuroscience | Ion Channels | Potassium Channels | Voltage-Gated Potassium Channels | Cell Signaling | Cancer

MabSelect™ PrismA

1 µg/ml was sufficient for detection of SAP97 on Rat Brain Membrane lysate.

1mg/mL

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

PBS pH 7.4, 0.02% Proclin 300, 50% glycerol

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Blue Ice or 4ºC

-20ºC

https://www.stressmarq.com/wp-content/uploads/SMC-631_SAP97_Antibody_G28_WB_Rat_brain-membrane-lysate_1.png | https://www.stressmarq.com/wp-content/uploads/SMC-631_SAP97_Antibody_G28_ICC-IF_Rat_Pheochromocytoma-cells_1.png | https://www.stressmarq.com/wp-content/uploads/SMC-631_SAP97_Antibody_G28_ICC-IF_Human_Neuroblastoma-cell-line-Kelly_1.png | https://www.stressmarq.com/wp-content/uploads/SMC-631_SAP97_Antibody_G28_WB_Human_Cervical-cancer-cell-line-HeLa_1.png | https://www.stressmarq.com/wp-content/uploads/SMC-631_SAP97_Antibody_G28_WB_Mouse_Brain-lysate_1.png

Detects N-terminal region of SAP97. Does not cross-react against PSD95, Chapsyn-110, or SAP102

1. E. I., Marshall, J., Aoki, C., de Silva, T., Montgomery, J. M., Garner, C. C., & Green, W. N. (2009) . SAP97 and CASK mediate sorting of NMDA receptors through a previously unknown secretory pathway. Nature Neuroscience, 12, 1011–1019. https://doi.org/10.1038/nn.2362 2. Kay, Y., Tsan, L., Davis, E. A., Tian, C., Décarie-Spain, L., Sadybekov, A., Pushkin, A. N., Katritch, V., Kanoski, S. E., & Herring, B. E. (2022) . Schizophrenia-associated SAP97 mutations increase glutamatergic synapse strength in the dentate gyrus and impair contextual episodic memory in rats. Nature Communications, 13, 798. https://doi.org/10.1038/s41467-022-28430-5 3. Saraceno, C., Marcello, E., Di Marino, D., Borroni, B., Claeysen, S., Perroy, J., Padovani, A., Tramontano, A., Gardoni, F., & Di Luca, M. (2014) . SAP97-mediated ADAM10 trafficking from Golgi outposts depends on PKC phosphorylation. Cell Death and Disease, 5, e1542. https://doi.org/10.1038/cddis.2014.492

Human | Mouse | Rat

Cell membrane, Endoplasmic reticulum membrane, Postsynaptic density, Synapse, Cell junction, Cytoplasm

IgG Kappa

Rat