SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antilichaam

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1) . The disease is the cause of the 2019–20 coronavirus outbreak (2) . The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP14 (ExoN) is an enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity (3,4,5) . It acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens (6,7,8) .

43740578

P0DTC1

YP_009725309.1

Virus

9187

Unconjugated

Primary Antibodies

Infectious Disease, COVID-19

SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is affinity chromatography purified via peptide column.

1 mg/mL

Predicted reactivity based on immunogen sequence: SARS-CoV NSP14 (ExoN) protein: (79%) .

SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.

Blue Ice

SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Antibody validated: SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can detect 2 ng of free peptide at 1 μ g/mL in ELISA.

ExoN

ORF1a polyprotein; ORF1ab polyprotein

Severe acute respiratory syndrome coronavirus 2

Gorbalenya. bioRxiv: 2020.

Hui et al. Int J Infect Dis. 2020;91:264-266.

Minskaia et. al. Proc. Natl. Acad. Sci. U.S.A 2006; 103:5108-5113

Bouvet et. al. PLoS Pathog. 2010; 6:E1000863-E1000863

Bouvet et. al. Proc. Natl. Acad. Sci. U.S.A. 2012;109:9372-9377

Denison et. al. RNA Biol. 2011;8:270-279

Smith et. al. PLoS Pathog. 2013;9:e1003565-e1003565

Agostini et. al. MBio 2018; 9:0-0

Proofreading exoribonuclease, Guanine-N7 methyltransferase, Non-structural protein 14, NSP14.

ELISA

1802476817

Liquid