SARS-CoV-2 (COVID-19) Nucleocapsid Antilichaam

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Virus

Recombinant SARS-CoV-2 Nucleocapsid

Unconjugated

Primary Antibodies

Infectious Disease, COVID-19

Purified by Protein A.

≥1 mg/mL

0.01M PBS (pH7.4) with 0.1% Proclin300

None

Blue Ice

Store at 4˚ C short term. Aliquot and store at -20˚ C long term. Avoid freeze-thaw cycles.

IgG

WB (1:500-1000), ELISA (1:5000-10000)

N

Nucleocapsid phosphoprotein

SARS-CoV-2

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WB, ELISA

Liquid