SARS-CoV-2 (COVID-19) Spike Antilichaam

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1) . The disease is the cause of the 2019–20 coronavirus outbreak (2) . The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3) . The spike protein is the major target for neutralizing antibodies and vaccine development (4) . The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5) . The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6) .

43740568

P0DTC2

QHD43416

Virus

3525

Unconjugated

Primary Antibodies

Infectious Disease, COVID-19

SARS-CoV-2 (COVID-19) Spike Antibody is affinity chromatography purified via peptide column.

1 mg/mL

Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (100%)

SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

SARS-CoV-2 (COVID-19) Spike antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

IgG

The Extracellular domain (ECD) is from aa 1 to 1208 (full length 1273aa) . Therefore, this antibody detects the transmembrane and cytoplasm domains at the C terminus, but does not detect the ECD (which is the region expressed in many commercially available spike proteins) . 3525 can be used for the detection of full length spike protein and spike protein in COVID-19 patient samples. All other applications and species not yet tested.

SARS-CoV-2 (COVID-19) Spike has one isoform (1273aa) .

S

Surface glycoprotein

Severe acute respiratory syndrome coronavirus 2

Gorbalenya. bioRxiv: 2020.

Hui et al. Int J Infect Dis. 2020;91:264-266.

Belouzard et al. Viruses. 2012;4 (6) :1011-33.

Lee et al. J Virol. 2006;80 (8) :4079-87.

Wan et al. J Virol. 2020.

Wrapp et al. Science. 2020.

32659620

32299776

32895985

33058948

32779733

32387855

32805515

33340034

SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

ELISA, WB, IHC, IF

1791269090

Liquid