SARS-CoV-2 RBD ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for SARS-CoV-2 RBD has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any SARS-CoV-2 RBD present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled SARS-CoV-2 RBD antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells.Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SARS-CoV-2 RBD bound in the initial step. The color development is stopped and the intensity of the color is measured.

RBD

Colorimetric

Quantitative

Serum, Plasma and Cell culture supernatant.

12.5 - 800 pg/mL

80-120%

4.92 pg/mL

2-8 °C

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.

For Research Use Only.