SARS-CoV-2 Spike Trimer Proteïne, zijn tag (KP.3) (MALS geverifieerd)

The spike protein of SARS-CoV-2 variant KP.3, a sublineage of Omicron JN.1, facilitates viral entry by binding the human ACE2 receptor through its receptor-binding domain. KP.3 carries characteristic mutations, including changes in the N-terminal and receptor-binding domains, that may enhance transmissibility and immune evasion. These alterations are under close surveillance for their potential impact on vaccine effectiveness, therapeutic antibody binding, and the ongoing evolution of SARS-CoV-2 in the global population.

SARS-CoV-2 Spike Trimer Protein, His Tag (KP.3) (SPN-S522e) is expressed from human 293 cells (HEK293) . It contains AA Val 16 -Pro 1213 (Accession # QHD43416.1 (T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q493E, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, D796Y, S939F, Q954H, N969K, V1104L, P1143L, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P) ) . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.7 kDa. The protein migrates as 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Val 16 - Pro 1213

Spike protein

Unconjugated

C-10xHis

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

1.0 EU per μg

90%

Powder

0.1 M Sodium citrate, pH5.5

137.7 kDa

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RT

-20°C

500ug*2

HEK293

SARS-CoV-2

QHD43416.1 (T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q493E, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, D796Y, S939F, Q954H, N969K, V1104L, P1143L, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)

Trehalose