SETDB1 Antilichaam - N-terminalgebied: HRP (ARP38998 P050-HRP)

SET domain, bifurcated 1

ESET, KG1T, KMT1E, TDRD21, H3-K9-HMTase4

9869

Q15047-2

Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETDB1

SETDB1 is a histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.

MDM2; HSP90AA1; UBC; SKIL; LRIF1; BARD1; APLP1; TCERG1; TRIM28; VIM; SUMO2; PML; CLSTN1; JARID2; tat; MZT2B; FAM118B; MRPL44; LUC7L2; MAP4K5; TSC22D1; TTR; TK1; SMN1; PCYT2; PSME1; GRB7; CDKN1A; CDK4; BID; ANXA7; ZNF274; HIST1H3A; ESCO1; AKT2; AKT1; PPARG

HRP: Horseradish Peroxidase

Polyclonal Antibody

WB

Affinity Purified

0.5 mg/ml

Cow: 92%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Rabbit: 85%; Rat: 86%

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

43kDa

Wet Ice

397

SETDB1

SETDB1

Rabbit

Histone-lysine N-methyltransferase SETDB1

PGCIGLDAATATVESEEIAELQQAVVEELGISMEELRHFIDEELEKMDCV