Shc1 (fosfo Tyr427) Antilichaam

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span

6464

P29353

NP_001123512.1

Human, Mouse, Rat

Shc1 (Phospho-Tyr427) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y (p) -V-N derived from Human Shc1.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

46, 52 kDa

This antibody detects endogenous level of Shc1 only when phosphorylated at tyrosine 427.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

SHC1

SHC (Src homology 2 domain containing) transforming protein 1

Homo sapiens

Trampont P, et al. (2006) Mol Cell Biol; 26 (23) : 9035-9044. Patrussi L, et al. (2005) Oncogene; 24 (13) : 2218-2228van der Geer P, et al. (1996) Curr Biol ; 6 (11) : 1435-1444

SHC, SHCA, PTP2C, SHPTP2, SH2 domain protein C1, SHC, SHC-transforming protein 1, Src homology 2 domain-containing-transforming protein C1

WB, IHC, IF

194239664

Liquid