Siglec-2 / CD22 Proteïne (aaa 176-687, His Tag)

CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR) -induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.

SIGLEC-2 Protein; SIGLEC2 Protein

Q32M46

Human

Human

His-Tag

See Datasheet send with the product

< 1.0 EU per μg protein as determined by the LAL method.

≥ 95 % as determined by SEC-HPLC.

1.Immobilized Anti-CD22 Antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human Siglec-2 / CD22 Protein (aa 176-687, His Tag) . The EC50 is 8-25 ng/mL. 2. Loaded Anti-Human CD22 Antibody, Human IgG1 on AHC Biosensor, can bind Recombinant Human Siglec-2/CD22 Protein (aa 176-687), His Tag with an affinity constant of 5.92 nM as determined in BLI assay (Sartorius Octet RED384) . 3. Monoclonal anti-Human Siglec-2 Antibody, human IgG1 captured on Protein A chip can bind Recombinant Human Siglec-2/CD22 Protein (aa 176-687), His Tag with an affinity constant of 2.147 nM as determined in an SPR assay (Biacore T200) .

523

Lyophilized

PBS, pH 7.4

See Datasheet

100 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells