SIK3 Antilichaam/zoutuitputtelijk kinase 3

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q9Y2K2

Human, Mouse, Rat

E.coli-derived human SIK3 recombinant protein (Position: E309-E1069) was used as the immunogen for the SIK3 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This SIK3 antibody is available for research use only.

After reconstitution, the SIK3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the SIK3 antibody should be determined by the researcher.

Western blot analysis of SIK3 using anti-SIK3 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HEL whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIK3 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected band size for SIK3 is at 145 kDa but is often observed as an ~150 kDa doublet representing differentially phosphorylated forms of SIK3 protein.