SIL-12 receptor beta-1 Fc

The IL‐12 structurally related family of heterodimeric proteins plays an important role in activating, regulating, and modulating the immune response. This family of proteins transmits its activity through receptors, which, when stimulated by ligand binding, generally activate JAK proteins that subsequently phosphorylate STAT transcription factors. IL‐12R β‐1 is a single pass membrane protein expressed on T cells, B cells, and NK cells. By itself, IL‐12R β‐1 binds to IL‐12 with low affinity, but when paired with IL‐12R β‐2, forms a high affinity IL‐12 receptor. IL‐12R β‐1 can also pair with IL‐23R to form a high affinity receptor for IL‐23. Recombinant Human sIL‐12 Receptor β‐1 Fc has a calculated molecular weight of 84.3 kDa and contains 758 amino acid residues, which consists of the extracellular domain of IL‐12R β‐1 fused to the Fc portion of human IgG and contains a C‐terminal His‐tag. Due to glycosylation, it migrates at an approximate molecular weight of 116 kDa, under reducing conditions.

Soluble IL‐12 receptor subunit beta‐1, soluble IL‐12 receptor beta‐1, soluble IL‐12R beta‐1, soluble IL12RB1, IL‐12R, CD212

3594

P42701

NP_001276952.1

NM_001290023.2

19p13.11

Human

Human

Fc-tag, C-terminal His-tag

CRTSECCFQD PPYPDADSGS ASGPRDLRCY RISSDRYECS WQYEGPTAGV SHFLRCCLSS GRCCYFAAGS ATRLQFSDQA GVSVLYTVTL WVESWARNQT EKSPEVTLQL YNSVKYEPPL GDIKVSKLAG QLRMEWETPD NQVGAEVQFR HRTPSSPWKL GDCGPQDDDT ESCLCPLEMN VAQEFQLRRR QLGSQGSSWS KWSSPVCVPP ENPPQPQVRF SVEQLGQDGR RRLTLKEQPT QLELPEGCQG LAPGTEVTYR LQLHMLSCPC KAKATRTLHL GKMPYLSGAA YNVAVISSNQ FGPGLNQTWH IPADTHTEPV ALNISVGTNG TTMYWPARAQ SMTYCIEWQP VGQDGGLATC SLTAPQDPDP AGMATYSWSR ESGAMGQEKC YYITIFASAH PEKLTLWSTV LSTYHFGGNA SAAGTPHHVS VKNHSLDSVS VDWAPSLLST CPGVLKEYVV RCRDEDSKQV SEHPVQPTET QVTLSGLRAG VAYTVQVRAD TAWLRGVWSQ PQRFSIEGGP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHHHH

< 0.1 ng/µg of protein (< 1EU/µg)

≥ 98% by SDS-PAGE gel and HPLC analyses.

Human IL-12 Receptor β-1 Fc activity is measured by its ability to bind to Human IL-12p70 in a functional ELISA.

758

Lyophilized

84.3 kDa

CHO cells