SIM2 Antilichaam / single-minded homolog 2 / BHLHE15

Single-minded homolog 2 is a protein that in humans is encoded by the SIM2 gene. This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q14190

Human, Monkey, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids Y337-R609) was used as the immunogen for the SIM2 antibody.

WB, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This SIM2 antibody is available for research use only.

After reconstitution, the SIM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the SIM2 antibody should be determined by the researcher.

Flow cytometry testing of fixed and permeabilized human PC-3 cells with SIM2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= SIM2 antibody.