SIRT2 cDNA

SIRT2 (NAD-dependent deacetylase sirtuin-2) is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIRT2 is a NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin.

Sirtuin 2, Sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2, SIR2L, NAD-dependent deacetylase sirtuin-2, SIR2, SIR2L2

19q13

Human

PATGen (puc19-derived cloning vector)

ATGGACTTCC TGCGGAACTT ATTCTCCCAG ACGCTCAGCC TGGGCAGCCA GAAGGAGCGT CTGCTGGACG AGCTGACCTT GGAAGGGGTG GCCCGGTACA TGCAGAGCGA ACGCTGTCGC AGAGTCATCT GTTTGGTGGG AGCTGGAATC TCCACATCCG CAGGCATCCC CGACTTTCGC TCTCCATCCA CCGGCCTCTA TGACAACCTA GAGAAGTACC ATCTTCCCTA CCCAGAGGCC ATCTTTGAGA TCAGCTATTT CAAGAAACAT CCGGAACCCT TCTTCGCCCT CGCCAAGGAA CTCTATCCTG GGCAGTTCAA GCCAACCATC TGTCACTACT TCATGCGCCT GCTGAAGGAC AAGGGGCTAC TCCTGCGCTG CTACACGCAG AACATAGATA CCCTGGAGCG AATAGCCGGG CTGGAACAGG AGGACTTGGT GGAGGCGCAC GGCACCTTCT ACACATCACA CTGCGTCAGC GCCAGCTGCC GGCACGAATA CCCGCTAAGC TGGATGAAAG AGAAGATCTT CTCTGAGGTG ACGCCCAAGT GTGAAGACTG TCAGAGCCTG GTGAAGCCTG ATATCGTCTT TTTTGGTGAG AGCCTCCCAG CGCGTTTCTT CTCCTGTATG CAGTCAGACT TCCTGAAGGT GGACCTCCTC CTGGTCATGG GTACCTCCTT GCAGGTGCAG CCCTTTGCCT CCCTCATCAG CAAGGCACCC CTCTCCACCC CTCGCCTGCT CATCAACAAG GAGAAAGCTG GCCAGTCGGA CCCTTTCCTG GGGATGATTA TGGGCCTCGG AGGAGGCATG GACTTTGACT CCAAGAAGGC CTACAGGGAC GTGGCCTGGC TGGGTGAATG CGACCAGGGC TGCCTGGCCC TTGCTGAGCT CCTTGGATGG AAGAAGGAGC TGGAGGACCT TGTCCGGAGG GAGCACGCCA GCATAGATGC CCAGTCGGGG GCGGGGGTCC CCAACCCCAG CACTTCAGCT TCCCCCAAGA AGTCCCCGCC ACCTGCCAAG GACGAGGCCA GGACAACAGA GAGGGAGAAA CCCCAGTGA

SIRT2, SIR2, SIR2L, SIR2L2, SIRT2, ATGD0041-10 µg, ATGD0041-20 µg, ATGD0041-50 µg, ATGD0041-100 µg, ATGD0041-250 µg, ATGD0041-500 µg, ATGD0041-1 mg, ATGD0041-10, ATGD0041-20, ATGD0041-50, ATGD0041-100, ATGD0041-250, ATGD0041-500, ATGD0041-1

Store the plasmid at -20C.

Lyophilized

Cell Cycle

604480

NP_085096.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_030593.2

1059bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.