SKA3 Antilichaam

SKA3 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.

221150

Q8IX90

NP_659498

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Homeostasis

SKA3 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1313 - Human Testis Tissue Lysate

1 mg/mL

SKA3 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

SKA3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

SKA3

Spindle and kinetochore associated complex subunit 3

Homo sapiens

Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21.

Hanisch A, Sillje HHW, and Nigg EA. Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska EMBO J.2006; 25:5504-15.

Gaitanos TN, Santamaria A, Jeyaprakash AA, et al. Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf EMBO J.2009; 28:1442-52.

Daum JR, Wren JD, Daniel JJ, et al. Ska3 is required for spindle checkpoint silencing and maintenance of chromosome cohesion in mitosis. Curr. Biol.2009; 19:1467-72.

SKA3 Antibody: RAMA1, C13orf3, RAMA1, Spindle and kinetochore-associated protein 3

ELISA, WB

260763910

Liquid