SLC25A12 Antilichaam / ARALAR1

Western blot: 1:500-1:2000, Immunoprecipitation: 1:50

O75746

Human, Mouse, Rat

A synthesized peptide derived from human SLC25A12 was used as the immunogen for the SLC25A12 antibody.

30S67

WB, IP

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This SLC25A12 antibody is available for research use only.

Store the SLC25A12 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the SLC25A12 antibody should be determined by the researcher.

Western blot analysis of SLC25A12 using anti-SLC25A12 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human whole cell lysates, Lane 2: human LNCAP whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A12 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A single band is detected at ~70 kDa, running slightly below the ~75 kDa prediction. The shift is consistent with mitochondrial targeting-sequence cleavage and the known faster SDS-PAGE migration of the mature aralar1 mitochondrial carrier protein.