SMAD5 (NM 005903) Humane overexpressie Lysaat

Transient overexpression lysate of SMAD family member 5 (SMAD5), transcript variant 1

DWFC; JV5-1; MADH5

4090

NM_005903

5q31.1

HEK293T

C-Myc/DDK

TGF-beta signaling pathway

TGF-beta signaling pathway

Liquid

Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors

52.3 kDa

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

SMAD5

Human

NP_005894

HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.

https://www.ncbi.nlm.nih.gov/gene/4090

https://www.ncbi.nlm.nih.gov/nuccore/NM_005903

https://www.ncbi.nlm.nih.gov/nuccore/NP_005894