Snelle overdrachtsbuffer (10X) (Powder)

Quick, efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications

WB

Store at 25°C for 2 years.

Wet Transfer Protocol 1. Prepare membrane and filter paper for transfer: a. A blotting membrane and 3 pieces of filter paper should be cut to fit dimensions of the gel. Note: PVDF membranes must be pre-wetted according to the manufacturer’s instructions in 100% methanol prior to equilibration in transfer buffer. b. Equilibrate the membrane and filter paper in 1X Rapid Transfer Buffer for a minimum of 5 minutes. 2. Following protein electrophoresis, assemble the blotting sandwich following the manufacturer’s instructions for the transfer apparatus. 3. Place the blotting sandwich in a wet transfer tank filled with 1X Rapid Transfer Buffer. 4. Transfer constant current 300 - 400 mA at room temperature. Semi-Dry Transfer Protocol 1. Prepare membrane and filter paper for transfer: a. A blotting membrane and 3 pieces of filter paper should be cut to fit dimensions of the gel. Note: PVDF membranes must be pre-wetted according to the manufacturer’s instructions in 100% methanol prior to equilibration in transfer buffer. b. Equilibrate the membrane and filter paper in 1X Rapid Transfer Buffer for a minimum of 5 minutes. 2. Following electrophoresis, wash the gel for 2 minutes in deionized water. 3. Pre-equilibrate the gel in 1X Rapid Transfer Buffer for 5 minutes. 4. Assemble the blotting sandwich following the manufacturer’s instructions for the semi-dry transfer apparatus. 5. Transfer constant current 300 - 400 mA at room temperature.

Fast, efficient transfer in 10 - 30 minutes Compatible with standard wet and semi-dry transfer equipment Methanol-free, non-hazardous formulation Transfers to PVDF and nitrocellulose from poured or pre-cast gels