SNRPG cDNA

Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. Appears to function in the U7 snRNP complex that is involved in histone 3-end processing.

Sm-G, SMG

2p13.3

Human

PATGen (puc19-derived cloning vector)

ATGAGCAAAGCTCACCCTCCCGAGTTGAAAAAATTTATGGACAAGAAGTTATCATTGAAATTAAATGGTGGCAGACATGTCCAAGGAATATTGCGGGGATTTGATCCCTTTATGAACCTTGTGATAGATGAATGTGTGGAGATGGCGACTAGTGGACAACAGAACAATATTGGAATGGTGGTAATACGAGGAAATAGTATCATCATGTTAGAAGCCTTGGAACGAGTATAA

SNRPG, Sm-G, SMG, SNRPG, ATGD0250-10 µg, ATGD0250-20 µg, ATGD0250-50 µg, ATGD0250-100 µg, ATGD0250-250 µg, ATGD0250-500 µg, ATGD0250-1 mg, ATGD0250-10, ATGD0250-20, ATGD0250-50, ATGD0250-100, ATGD0250-250, ATGD0250-500, ATGD0250-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

603542

NP_003087.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_003096.2

231bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.