SOCS7 Antilichaam / Onderdrukker van cytokine signalering 7

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

O14512

Human

A synthesized peptide derived from human SOCS7 was used as the immunogen for the SOCS7 antibody.

30S97

WB, IHC

Affinity-chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This SOCS7 antibody is available for research use only.

Store the SOCS7 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the SOCS7 antibody should be determined by the researcher.

Western blot analysis of SOCS7 using anti-SOCS7 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS7 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A band is detected in the commonly cited 58-63 kDa range. This migration is consistent with endogenous SOCS7 and reflects differences in phosphorylation state and isoform usage, which modestly alter SDS-PAGE mobility.