SOX9 Antilichaam / SRY-box transcriptiefactor 9

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

P48436

Human, Mouse, Rat

E.coli-derived human SOX9 recombinant protein (Position: M1-Q496) was used as the immunogen for the SOX9 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This SOX9 antibody is available for research use only.

After reconstitution, the SOX9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the SOX9 antibody should be determined by the researcher.

Western blot analysis of SOX9 using anti-SOX9 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot detection of SOX9 shows a major band at ~75 kDa with a minor band at ~80-85 kDa across multiple human cell lines. Although the calculated mass is ~56 kDa, SOX9 commonly migrates larger, and the upper species likely reflects additional post-translationally modified forms.