Spleen Membrane Tumor Lysate
Kwaliteit
ISO Gecertificeerd
Levering
24-48 uur
Technische Specificaties
Human spleen tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human spleen tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚ C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated spleen tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated spleen tissue membrane protein is then Western analyzed by either GAPDH or β -actin antibody to confirm there is no signal or very weak signal.
Lysates
2 mg/mL or better
HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Dry Ice
Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Spleen membrane protein lysate is for use in Western blotting, 10 μ g to 20 μ g per lane is recommended for mini gel.
WB
Human
Liquid
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