SPT2 Antilichaam

SPT2 Antibody: Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.

9517

O15270

NP_004854

Human, Mouse

6305

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 1) shows similar SPT2 expression profile in human cell lines detected by two independent anti-SPT2 antibodies that recognize different epitopes, 6305 against C-terminus domain and 64-238 against central domain. SPT2 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines6305 64-238

Homeostasis

SPT2 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1282 - 3T3 (NIH) Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Rat (100%) .

SPT2 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

SPT2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

SPT2 antibody is predicted to not cross-react with SPT1.

Human SPT2 has one isoform (562aa, 63kD) . Mouse SPT2 has one isoform (560aa, 63kD) and Rat SPT2 also has one isoform (560aa, 63kD) . 6305 can detect human, mouse and rat.

SPTLC2

Cat. No. 17-201 - 3T3/BALB Cell Slide

Cat. No. 1204 - K562 Cell Lysate

Cat. No. 1219 - MCF7 Cell Lysate

Cat. No. 1220 - SK-N-SH Cell Lysate

Serine palmitoyltransferase, long chain base subunit 2

Homo sapiens

Batheja et al. J. Histochem. & Cytochem. 2003; 51:687-96.

Nagiec et al. Gene 1996; 177:237-41

Yasuda et al. J. Biol. Chem. 2003; 278:4176-83.

Rotthier et al. Am. J. Hum. Genet. 2010; 87:513-22.

SPT2 Antibody: LCB2, SPT2, HSN1C, LCB2A, NSAN1C, hLCB2a, KIAA0526, LCB2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, LCB 2

ELISA, WB, ICC, IF

4758668

Liquid