SQSTM1 Antilichaam

SQSTM1 Antibody: SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-κ B by TNF-α , nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.

8878

Q13501

Q13501

Human, Mouse, Rat

Unconjugated

Primary Antibodies

KO Validation (Figure 1) : Anti-SQSTM1 antibodies (5449) specificity was further verified by SQSTM1 specific knockout. SQSTM1 signal was not detected in SQSTM1 knockout HEK293T cells as compared to that in control wild type cells. KO Validation5449) Induced Expression Validation (Figure 2) : Raw 264.7 cells were treated with LPS (0.3 µg /mL) for different time period (0-24 hrs) . SQSTM1 expression detected by anit-SQSTM1 antibodies (5449) was induced by LPS treatment and the expression levels were increased gradually over the period of 24 hrs. Induced Expression Validation5449

Homeostasis

SQSTM1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1306 - Human Spleen Tissue Lysate

1 mg/mL

SQSTM1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

SQSTM1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human SQSTM1 has two isoforms, including isoform 1 (440aa, 47.7kD) and isoform 2 (356aa, 38.6kD) . Mouse SQSTM1 also has two isoforms, including isoform 1 (442aa, 48.2kD) and isoform 2 (404aa, 44.2kD) . Rat SQSTM1 has three isoforms, including isoform 1 (439aa, 47.7kD), isoform 2 (412aa, 45kD) and isoform 3 (234aa, 26.1kD) . 5449 can detect the isoforms of human mouse and rat except the shortest rat isoform 3.

SQSTM1

Cat. No. 1204 - K562 Cell Lysate

Cat. No. 1201 – HeLa Cell Lysate

Cat. No. 1203 – A549 Cell Lysate

Cat. No. 1282 – 3T3/NIH Cell Lysate

Sequestosome 1

Homo sapiens

Seibenhener et al. Mol. Cell. Biol.2004; 24:8055-68.

Hocking et al. Hum. Mol. Genet.2002; 11:2735-9.

Rousiere et al. Best Pract. Res. Clin. Rheumatol.2003; 17:1019-41.

Layfield et al. Biochem. Soc. Trans.2004; 32:728-30.

SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP

ELISA, WB, IHC-P, IF

74735628

Liquid