SRANK League of Antibody

SRANK Ligand Antibody: The receptor activator of NF-κ B ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis. This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.

8600

O14788

NP_003692

Human, Mouse, Rat

3963

Unconjugated

Primary Antibodies

Recombinant Protein Test (Figure 3) : Anti-sRANK-L antibodies (3963) detected human sRANK-L recombinant protein at different concentrations. Recombinant Protein Test3963 Induced expression validation (Figure 6) : sRANK-L expression detected by anit-sRANK-L antibodies (3963) was up-regulated after 18hr by extraction of upper jaw molars in rat osteogenic cells. Induced expression validation3963

Apoptosis

SRANK Ligand Antibody is affinity chromatography purified via peptide column.

Cat. No. 1464 - Rat Liver Tissue Lysate

1 mg/mL

SRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

SRANK Ligand antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human sRANK-L has 3 isoforms, including isoform 1 (317aa, 35.5kD), isoform 2 (244aa, 27.7kD) and isoform 3 (270aa, 30.5kD) . Mouse sRANK-L also has 3 isoforms, including isoform 1 (316aa, 35kD), isoform 2 (287aa, 32.3kD) and isoform 3 (199aa, 22.2kD) . Rat sRANK-L has one isoform (318aa, 38.4kD) . 3963 can detect human, mouse and rat.

TNFSF11

Cat. No. 1304 - Human Liver Tissue Lysate

Cat. No. 10-201 - Human Liver Tissue Slide

Cat. No. 1404 - Mouse Liver Tissue Lysate

Tumor necrosis factor (ligand) superfamily, member 11

Homo sapiens

Wong, et al. J. Biol. Chem. 1997; 272:25190-4.

Kong et al. Nature 1999; 397:315-23.

Weitzmann et al. Blood 2000; 96:1873-8.

Bharti et al. J. Biol. Chem. 2004; 279:6065-76.

PMID: 29226261.

SRANK Ligand Antibody: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF

ELISA, WB, IHC-P

4507595

Liquid