STAM2 Antilichaam / Signaal transducerend adaptormolecuul 2

ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells

O75886

Human, Mouse, Rat

E.coli-derived human STAM2 recombinant protein (Position: K134-L525) was used as the immunogen for the STAM2 antibody.

ELISA, IF, ICC, WB, FACS

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This STAM2 antibody is available for research use only.

After reconstitution, the STAM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the STAM2 antibody should be determined by the researcher.

Immunofluorescent staining of STAM2 using anti-STAM2 antibody (red) and anti-Beta Tubulin antibody (green) . STAM2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-STAM2 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and DyLight 488 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used. U2OS cells were stained with STAM2 antibody (red) and Beta-tubulin antibody (green) . STAM2 displays cytoplasmic punctate staining, concentrated in the perinuclear region consistent with its association with early/sorting endosomes. The vesicular STAM2 signal partially aligns with the microtubule network, reflecting its role in endosome trafficking along microtubule tracks.