STAT1 (fosfo Tyr701) Antilichaam

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs) . Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-γ -activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

6772

P42224

NP_009330.1

Human, Mouse, Rat

STAT1 (Phospho-Tyr701) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y (p) -I-K) derived from Human STAT1.

Unconjugated

Primary Antibodies

Phospho-Specific, Apoptosis, Cancer , Inflammasomes

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

91 kDa

This antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

STAT1

Signal transducer and activator of transcription 1, 91kDa

Homo sapiens

Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Durbin J E, et al. (1996) Cell. 84: 443-450. Meraz M A, et al. (1996) Cell. 84: 431-442. Wakao H, et al. (1994) EMBO J. 13: 2182-2191. Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258 Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.

CANDF7, ISGF-3, STAT91, BHLHE39, Transcription factor ISGF-3 components p91/p84

WB, IHC

6274552

Liquid