Streptavidin antilichaam

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2.

Human

Recombinant Streptavidin (25-183aa) purified from E. coli

1C2

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Streptavidin, AST0904-10 µg, AST0904-20 µg, AST0904-50 µg, AST0904-100 µg, AST0904-250 µg, AST0904-500 µg, AST0904-1 mg, AST0904-10, AST0904-20, AST0904-50, AST0904-100, AST0904-250, AST0904-500, AST0904-1

Mark Howarth, et al. (2006) Nature Methods. 3:267-73.; ; Holmberg A., et al. (2005) Electrophoresis. 26 (3) :501-10.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Protein Marker

CAA27265

P22629

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Streptavidin antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Streptavidin protein