SUPT4H1 cDNA

SUPT4H1 encodes the small subunit of DRB (5, 6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene.

SPT4, SPT4H, SUPT4H

17q22

Human

PATGen (puc19-derived cloning vector)

ATGGCCCTGG AGACGGTGCC GAAGGACCTG CGGCATCTGC GGGCCTGTTT GCTGTGTTCG CTGGTCAAGA CTATAGACCA GTTTGAATAT GATGGTTGTG ACAATTGTGA TGCATATCTA CAAATGAAGG GTAACCGAGA GATGGTATAT GACTGCACTA GCTCTTCCTT TGATGGAATC ATTGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGAGT CAGTAACTTT AAGCCAGGTG TATATGCGGT GTCAGTCACT GGTCGCCTGC CCCAAGGAAT CGTGCGGGAG CTGAAAAGTC GAGGAGTGGC CTACAAATCC AGAGACACAG CTATAAAGAC CTAG

SUPT4H1, SPT4, SPT4H, SUPT4H, SUPT4H1, ATGD0094-10 µg, ATGD0094-20 µg, ATGD0094-50 µg, ATGD0094-100 µg, ATGD0094-250 µg, ATGD0094-500 µg, ATGD0094-1 mg, ATGD0094-10, ATGD0094-20, ATGD0094-50, ATGD0094-100, ATGD0094-250, ATGD0094-500, ATGD0094-1

Store the plasmid at -20C.

Lyophilized

Epigenetics and Nuclear Signaling

603555

NP_003159.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_003168.2

354bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.