Survivon Antilichaam

Survivin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.

332

O15392

NP_001159

Human

2235

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar Survivin expression profile in human cell lines detected by two independent anti-Survivin antibodies that recognize different epitopes, 2233 against N-terminus domain and 2235 against C-terminus domain. Survivin proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2233 2235 Recombinant Protein Test (Figure 6- 8) : Anti-Survivin antibodies (2235) detected human Survivin recombinant protein directly or in sera from cancer patients. Recombinant Protein Test2235

Apoptosis, Cancer

Survivin Antibody is affinity chromatography purified via peptide column.

Cat. No. 1206 - MOLT4 Cell Lysate

1 mg/ml

Predicted species reactivity based on immunogen sequence: Pig: (92%), Bovine: (79%), Mouse: (71%), Rat: (71%)

Survivin Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Survivin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human Survivin has 7 isoforms, including isoform 1 (142aa, 16kD), isoform 2 (165aa, 19kD), isoform 3 (137aa, 16kD), isoform 4 (120aa, 14kD), isoform 5 (117aa, 14kD), isoform 6 (78aa, 9kD) and isoform 7 (74aa, 8.5kD) . Mouse Survivin has 3 isoforms, including isoform 1 (140aa, 16kD), isoform 2 (121aa, 14kD) and isoform 3 (40aa, 4.7kD) . Rat Survivin has one isoform (142aa, 17kD) . 2235 can detect human and possibly mouse and rat.

BIRC5

Cat. No. 17-006 - MOLT-4 Cell Slide

Cat. No. 1201 - HeLa Cell Lysate

Cat. No. 1204 - K562 Cell Lysate

Cat. No. 1224 - Daudi Cell Lysate

Baculoviral IAP repeat-containing 5

Homo sapiens

Ambrosini et al. Nat Med 1997;3:917-21.

Li et al. Nature 1998;396:580-4.

Kobayashi et al. Proc Natl Acad Sci USA 1999;96:1457-62 (WD0600) .

17067715

15896923

20523766

Survivin Antibody: API4, EPR-1, API4, IAP4, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4

ELISA, WB, ICC, IF

59859878

Liquid