Survivon Antilichaam / BIRC5

The baculovirus protein p35 inhibits virally induced apoptosis of invertebrate and mammalian cells and may function to impair the clearing of virally infected cells by the host s immune system. This is accomplished at least in part by its ability to block both TNF- and FAS-mediated apoptosis through the inhibition of the ICE family of serine proteases. Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino terminal baculovirus IAP repeat (BIR) motif and a carboxy terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FAS-mediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivin-microtubule interactions occurs.

Western blot: 2-4 µg/mL

O15392

Human

A portion of amino acids 1-142 from human BIRC5 protein was used as the immunogen for the Survivin antibody.

BIRC5/9071

WB

Protein G affinity

Purified

This Survivin antibody is available for research use only.

Aliquot the Survivin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the Survivin antibody should be determined by the researcher.

Nucleus, Cytoplasm

Western blot testing of human 293T cell lysate with BIRC5 antibody (clone BIRC5/9071) . Predicted molecular weight ~16 kDa.