SW480 Lysaat

SW480 cells was established from a primary adenocarcinoma of the colon in a 50 year old male. SW480 cells are epithelial like in morphology and used as an in vitro model for colorectal cancer to study tumor markers, CEA production, biochemistry of tumorigenicity, pharmacodynamics, hormone sensibility and as a positive control for the nuclear protein Beta catenin.

Human

WB

2 mg/mL (determined by BCA assay)

SW480, ATGL0011-10 µg, ATGL0011-20 µg, ATGL0011-50 µg, ATGL0011-100 µg, ATGL0011-250 µg, ATGL0011-500 µg, ATGL0011-1 mg, ATGL0011-10, ATGL0011-20, ATGL0011-50, ATGL0011-100, ATGL0011-250, ATGL0011-500, ATGL0011-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : SW480 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody