Synaptotagmin (fosfo Ser309) Antilichaam

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis. Moreover recent studies indicate that the protein also plays a key role in endocytosis. Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission.

25716

P21707

P21707

Human, Mouse, Rat

Synaptotagmin (Ser309) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Ser309 of synaptotagmin.

Unconjugated

Primary Antibodies

Neuroscience, Phospho-Specific

Affinity Purified

Batch dependent

Phospho-Specific

Blue Ice

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

60/62

Synaptotagmin antibody is specific for Mr 60 - 62k synaptotagmin phosphorylated at Ser309.

Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Syt1

Synaptotagmin I

Rattus norvegicus

Poskanzer, K.E., Marek, K.W., Sweeney, S.T. and Davis, G.W., "Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo, " Nature (London) 426 (2003) 559 - 563.

Wang, C.T., Lu, J.C., Bai, J.H., Chang, P.Y., Martin, T.F.J., Chapman, E.R. and Jackson, M.B. "Different domains of synaptotagmin control the choice between kiss-and-run and full fusion, " Nature (London) 424 (2003) 943 - 947.

Fernandez-Chacon, R., Konigstorfer, A., Gerber, S.H., Garcia, J., Matos, M.F., Stevens, C.F., Brose, N., Rizo, J., Rosenmund, C. and Sudhof, T.C., "Synaptotagmin I functions as a calcium regulator of release probability, " Nature (London) 410 (2001) 41 - 49.

Hilfiker, S., Pieribone, V.A., Nordstedt, C., Greengard, P., and Czernik, A.J., "Regulation of synaptotagmin I phosphorylation by multiple protein kinases, " J. Neurochem. 73 (1999) 921 - 932.

WB, IHC

94730428

Liquid