TACSTD2-eiwit (C-His, mens)

TACSTD2

EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

> 96 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

1.Loaded Anti-TROP2 (Research Grade Sacituzumab Biosimilar) on ProA Biosensor, can bind Recombinant Human TROP2 Protein, His Tag (Cat.No.10428-H08H) with an affinity constant of 1.64 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Immobilized Anti-TROP2 (Research Grade Sacituzumab Biosimilar) at 2μg/mL (100μL/well) can bind Recombinant Human TROP2 / TACSTD2 Protein (His Tag) (Cat: 10428-H08H), the EC50 is 1.6-4.8 ng/mL (Routinely tested) . 3.Anti-TROP2 (Research Grade Datopotamab Biosimilar) captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Human TROP2 / TACSTD2 Protein (His Tag) (Cat: 10428-H08H) with an affinity constant of 25.100 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 4.Immobilized Recombinant Human TROP2 / TACSTD2 Protein (His Tag) (Cat: 10428-H08H) at 1 μg/mL (100 μL/well) can bind Anti-TROP2 (Research Grade Datopotamab Biosimilar), the EC50 is 0.5-2.0 ng/mL (QC tested) .

Met1-Thr274

The recombinant human TROP2 comprises 259 amino acids and has a predicted molecular mass of 29.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TROP2 is approximately 42-48 kDa due to glycosylation.

Human