Tau 2N Antilichaam: RPE

Tau is a microtubule-associated protein encoded by the MAPT gene, which undergoes complex alternative splicing to produce six isoforms in the adult human brain. These isoforms differ by the presence of zero, one, or two N-terminal inserts (0N, 1N, 2N) and either three (3R) or four (4R) microtubule-binding repeat domains. The 2N isoform, characterized by two N-terminal inserts, exists in both 2N3R and 2N4R variants. These isoforms are involved in modulating tau’s interaction with microtubules and other cellular components. The 3R/4R ratio is tightly regulated in the healthy brain but becomes disrupted in tauopathies. For example, Alzheimer’s disease (AD) exhibits a mixed 3R/4R tau pathology, while other disorders such as Pick’s disease or progressive supranuclear palsy show isoform-specific accumulation. Tau 2N isoforms are of particular interest in neurodegenerative research due to their distinct expression patterns and potential contributions to disease-specific tau aggregation. Tools that selectively detect human 2N isoforms—such as isoform-specific antibodies like StressMarq's Tau 2N Antibody—are valuable for distinguishing pathological tau species and for developing targeted diagnostics and therapeutics. Our human Tau 2N3R/4R polyclonal antibody has demonstrated specificity to human 2N isoforms and does not detect rodent isoforms. Understanding the role of Tau 2N in isoform imbalance and aggregation provides critical insight into the molecular mechanisms driving neurodegeneration.

Rabbit Anti-Human Tau Polyclonal

Tau, 2N4R Tau, 2N3R Tau, pTau, MAPT, Neurofibrillary tangle protein, Tau-Human

12352203

Non-hazardous

Non-hazardous

P10636-5

Tau 2N3R/4R peptide

RPE

Cytoplasmic expression mainly in CNS, peripheral nerves and renal tubules.

Polyclonal

WB

Neuroscience | Neurodegeneration | Parkinson's Disease | ALS Disease | Alzheimer's Disease

Affinity Purified

A 1:1000 dilution of SPC-813 was sufficient for detection of Tau 2N3R/4R spliceforms (range tested 2ug – 0.063ug) .

1mg/ml

WB (1:1000) ; optimal dilutions for assays should be determined by the user.

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

1. Goedert, M., Spillantini, M. G., Jakes, R., Rutherford, D., & Crowther, R. A. (1989) . Multiple isoforms of human microtubule-associated protein tau: sequences and localization in neurofibrillary tangles of Alzheimer's disease. Neuron, 3 (4), 519–526. DOI: 10.1016/0896-6273 (89) 90210-9 2. Spillantini, M. G., & Goedert, M. (2013) . Tau pathology and neurodegeneration. The Lancet Neurology, 12 (6), 609–622. DOI: 10.1016/S1474-4422 (13) 70090-5 3. Kovacs, G. G. (2017) . Tauopathies. Handbook of Clinical Neurology, 145, 355–368. DOI: 10.1016/B978-0-12-802395-2.00025-3

Blue Ice or 4ºC

Conjugated antibodies should be stored according to the product label

https://www.stressmarq.com/wp-content/uploads/Antibody-MSDS.pdf

Detects Tau 2N3R/4R spliceforms

Human

Plasma membrane

Human