TCIRG1 Antilichaam / T-cel immuunregulator 1

Immunohistochemistry: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q13488

Human

E.coli-derived human TCIRG1 recombinant protein (Position: Q48-D680) was used as the immunogen for the TCIRG1 antibody.

IHC, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This TCIRG1 antibody is available for research use only.

After reconstitution, the TCIRG1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the TCIRG1 antibody should be determined by the researcher.

Immunohistochemical staining of TCIRG1 using anti-TCIRG1 antibody. TCIRG1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TCIRG1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate. Tonsil section shows scattered cytoplasmic/vesicular staining in macrophages and histiocytes within the germinal centers, consistent with TCIRG1 expression in antigen-presenting phagocytes.