TCOF1 Antilichaam / Treacle proteïne

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q13428

Human, Mouse, Rat

E.coli-derived human TCOF1 recombinant protein (Position: M1-K1340) was used as the immunogen for the TCOF1 antibody.

WB, ICC/IF, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This TCOF1 antibody is available for research use only.

After reconstitution, the TCOF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the TCOF1 antibody should be determined by the researcher.

Nuclear, Nucleolar

Immunofluorescent staining of TCOF1 using anti-TCOF1 antibody (green) and anti-Beta Tubulin antibody (red) . TCOF1 was detected in an immunocytochemical section of human SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-TCOF1 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.